The FLIPR assay was carried out on FDSS/µMobile with ligand addition at 10?s for the binding assay and mAb1/isotype addition at ten?s accompanied by hCCL1 addition at 300?s for that Level of competition binding assay, with a total checking of five hundred?s. The excitation and emission wavelengths have been established at 485?nm and 525?nm, respec… Read More